CHEM 3322 – Ultra High Pressure Liquid Chromatography Mass Spectrometry

CHEM 3322 – Instrumental Methods of Analysis

Ultra High Pressure Liquid Chromatography Mass Spectrometry

Some general guidance on Laboratory Reports:

Format, Guidance and Grading Criteria Reports should be 1.5 lines spaced. Word and page limits should be maintained.

Abstract: (Maximum 200 words) Summarize in a concise paragraph the purpose of the report, data presented and major conclusions. What was done, why was it done, how was it done, what were the results and what conclusion(s) can be reported? Analytical abstracts must contain actual values when available. Read a few abstracts from published papers for additional guidance. Introduction: (1 page max) • Define the subject of the report: “Why was this study performed?” • What is the significance and relevance? In other words, why did we do this? • Outline scientific purpose(s) and/or objective(s): “What are the specific hypotheses and the experimental design for investigation?” Materials and Methods: (2 page max) • List chemicals used, any preparation required, who made them (vendor) and their concentration, purity and lot number where appropriate. • List all equipment used (instrumentation, columns etc.). Listing common equipment like glassware is not required. • Provide step-by-step procedure with enough detail for the reader to understand the experiment. Make sure to note weights, volumes, and any special glassware and equipment where appropriate. Results: (Page length as needed) • Concentrate on general trends and differences and not on trivial details. • Summarize the data from the experiments without discussing their implications • Organize data into tables, figures, graphs, etc. as needed. • Provide titles for all figures and tables; include a legend explaining symbols, abbreviations, or special methods if necessary. • Number figures and tables separately and refer to them in the text by their number, i.e. 1. Figure 1 shows that the activity…. 2. The activity decreases after five minutes (fig. 1) Discussion: (2 pages max) • Interpret the data; do not restate the results • Relate results to existing theory and knowledge • Explain the logic that allows you to accept or reject your original hypotheses • Speculate if necessary but identify it as such • Provide an explanation for poor or unexpected results. Put some thought to what may have caused these results Conclusions: (1 Paragraph) • Summarize the laboratory exercise and final outcomes Additional Guidance Length Reports are typically 4-8 pages in length including the title, data and references. Rarely if ever below 5 pages and occasionally above 8-10 pages if a significant amount of data/chromatograms are requested. Do not ever exceed 15 pages per report. Double spacing, large font and other ‘creative’ efforts to make the report appear longer are not impressive to the reviewer. Great reports are not necessarily long reports. Brevity in scientific writing is an important skill to master. General Appropriate grammar and readability of reports is required. If you have concerns with your ability to write effectively in English you must seek help from outside of this course. Please let me know if you have any questions. Abstracts for analytical reports should contain actual values/results and should not exceed 200 words. I want to know what you did in the experimental section, not just cut/paste from the lab manual and use of words such as approximately to describe amounts such as volumes and weights. Lab reports must be written in MS Word and submitted electronically through TurnItIn in Blackboard. Please use the convention M8G4Hall (Module #, Group #, Last Name) when naming files. Some guidance on point values and issues to look out for with laboratory reports: Note: This list is not all-inclusive but represents some of the most common errors and resulting point deductions. 1-point deductions: 1-point, Typos/Misspellings/Incorrect language, grammar 1-point, use of colloquial/non-scientific language 1-point, name and module number not provided on report 1-point, proper labeling of figures below and tables above 1-point, proper referencing 1-point, minor technical issues 1-point, incorrect filename convention 3-point deductions: 3-points, Questions must be written out and answered in a defined section of the report Variable Point Deductions: 1-5points, the manufacturer/model of all instruments as well as solutions prepared or provided must be included in the materials/methods section. Whenever possible organize consumables into tables or bullet points rather than embedded within a paragraph. 3-5points, Moderate to significant technical issues/concerns 3-5points, 200 word limit for abstract. <100 = -3, <50 = -5, >200 = -3, >250 = -5 3-5points, discretionary. Limited discussion. Lacking pertinent information.

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